So, we want to transfer it onto a filter. And what the filter willīasically allow us to do is it allow us to visualize 'cause this gel is very flimsy. So the next step, step number three is basically we're gonna take this gel and we're gonna transfer it to a filter. So now, we've got this gel and we've got the DNA fragments separated by size on this gel. So, we're gonna have theseįragments separated like so. Gonna move down the gel and they're gonna basically be separated based on size and based on charge. So, let's imagine that this is the gel and we add the DNA fragments So, we're gonna take these DNA fragments and we're gonna run them on a gel. But basically, the gel electrophoresis will help us separate these DNA fragments based on size and based on charge. And I made a video on gel electrophoresis if you want to refresh, you can watch that video. So, specifically we're gonna do a gel electrophoresis, 'electrophoresis' on these DNA fragments. Now Step 2, what do we do? Well, what we're gonna do is we're gonna take all these tiny little DNA fragments and we're gonna run them on the gel. And that will result in lots of these smaller pieces of DNA. We're gonna expose it to enzymes that will basically cleave the DNA in a whole bunch of different parts. So, we got this big strand and we're gonna cut it up. So, we're gonna take this big old strand. So, 'take the DNA and cleave it.' So, let me draw that out. So Step 1, what we're gonna do is we're gonna take this DNA and we're gonna cleave this. And we'll break it up intoĪ couple of different steps. Now, in order to figure out whether or not Gene A is inside this cup, basically we have to do this process known as a Southern Blot. If it's inside of this long piece of DNA. I'm interested in Gene A and I want to see if Gene A is inside of this cup. And there's just lotsĪnd lots of those DNA and let's imagine that I'm specifically interested in one gene. So it's got just a wholeīunch of DNA inside. So let's imagine that we have a cup and it's filled with DNA. So, a Southern Blot basically allows you to visualize a specific piece of DNA that you're interested in. Gonna be talking about something known as a Southern Blot.

Palindromic sequence mcat